dart enzyme sequences (Addgene inc)
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dart enzyme sequences
Dart Enzyme Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+apobec1+yth/pCMV-APOBEC1-YTH+(Plasmid+%23131636)/bio_rxiv__2025__05__02__651974-220-0-4
Average 93 stars, based on 8 article reviews
Dart Enzyme Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+apobec1+yth/pCMV-APOBEC1-YTH+(Plasmid+%23131636)/bio_rxiv__2025__05__02__651974-220-0-4
Average 93 stars, based on 8 article reviews
dart enzyme sequences - by Bioz Stars,
2026-09
93/100 stars
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Sequencing:Article Title: scDART-seq reveals distinct m 6 A signatures and mRNA methylation heterogeneity in single cells. Article Snippet: Cloning and stable cell line creation The lentiviral vector TLCV2 (gift from Adam Karpf, Addgene #87360) was used as the backbone for the lentiviral expression construct used to generate TLCV2-APOBEC1-YTH and TLCV2-APOBEC1-YTHmut plasmids, which were used to create stable Molecular Cell 82, 868–878.e1–e10, February 17, 2022 e3 cell lines. .. To clone TLCV2-APOBEC1-YTH and TLCV2-APOBEC1-YTHmut, the CAS9-FLAG sequence in TLCV2 was replaced with the APOBEC1-YTH (or APOBEC1-YTHmut for control cell lines) cassette from Article Title: In situ visualization of m 6 A sites in cellular mRNAs Article Snippet: .. To generate TLCV2-APO1-YTH, the APO1-YTH transgene was amplified from Control:Article Title: scDART-seq reveals distinct m 6 A signatures and mRNA methylation heterogeneity in single cells. Article Snippet: Cloning and stable cell line creation The lentiviral vector TLCV2 (gift from Adam Karpf, Addgene #87360) was used as the backbone for the lentiviral expression construct used to generate TLCV2-APOBEC1-YTH and TLCV2-APOBEC1-YTHmut plasmids, which were used to create stable Molecular Cell 82, 868–878.e1–e10, February 17, 2022 e3 cell lines. .. To clone TLCV2-APOBEC1-YTH and TLCV2-APOBEC1-YTHmut, the CAS9-FLAG sequence in TLCV2 was replaced with the APOBEC1-YTH (or APOBEC1-YTHmut for control cell lines) cassette from Generated:Article Title: Improved Methods for Deamination-Based m 6 A Detection. Article Snippet: DART protein variants used for cellular DART-seq (A3A-YTH, A3C-YTH, huAPO1-YTH, AID-YTH, rZDD-YTH, APO1YTHDF1, APO1-YTHDF1(D401N), and APO1-YTHD422N), were cloned into the pCMV-APOBEC1-YTH plasmid (Addgene #131636) in place of APOBEC1 or the YTH domain as indicated using Gibson Assembly (NEB). .. ADARcd-YTHD422N and ADARcd-YTHmut plasmids were generated by replacing APOBEC1 from the Article Title: Improved Methods for Deamination-Based m 6 A Detection Article Snippet: DART protein variants used for cellular DART-seq (A3A-YTH, A3C-YTH, huAPO1-YTH, AID-YTH, rZDD-YTH, APO1-YTH DF1 , APO1-YTH DF1(D401N) , and APO1-YTH D422N ), were cloned into the pCMV-APOBEC1-YTH plasmid (Addgene #131636) in place of APOBEC1 or the YTH domain as indicated using Gibson Assembly (NEB). .. ADARcd-YTH D422N and ADARcd-YTH mut plasmids were generated by replacing APOBEC1 from the Mutagenesis:Article Title: Improved Methods for Deamination-Based m 6 A Detection. Article Snippet: DART protein variants used for cellular DART-seq (A3A-YTH, A3C-YTH, huAPO1-YTH, AID-YTH, rZDD-YTH, APO1YTHDF1, APO1-YTHDF1(D401N), and APO1-YTHD422N), were cloned into the pCMV-APOBEC1-YTH plasmid (Addgene #131636) in place of APOBEC1 or the YTH domain as indicated using Gibson Assembly (NEB). .. ADARcd-YTHD422N and ADARcd-YTHmut plasmids were generated by replacing APOBEC1 from the Article Title: Improved Methods for Deamination-Based m 6 A Detection Article Snippet: DART protein variants used for cellular DART-seq (A3A-YTH, A3C-YTH, huAPO1-YTH, AID-YTH, rZDD-YTH, APO1-YTH DF1 , APO1-YTH DF1(D401N) , and APO1-YTH D422N ), were cloned into the pCMV-APOBEC1-YTH plasmid (Addgene #131636) in place of APOBEC1 or the YTH domain as indicated using Gibson Assembly (NEB). .. ADARcd-YTH D422N and ADARcd-YTH mut plasmids were generated by replacing APOBEC1 from the Amplification:Article Title: Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing Article Snippet: ALYREF and YBX1 expression plasmids were purchased from MIAOLING BIOLOGY ( http://www.miaolingbio.com/ ), and the ALYREF and YBX1 fractions were then amplified using specific primer. .. The ALYREF and YBX1 portions were amplified using Article Title: In situ visualization of m 6 A sites in cellular mRNAs Article Snippet: .. To generate TLCV2-APO1-YTH, the APO1-YTH transgene was amplified from Article Title: Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing Article Snippet: ALYREF and YBX1 expression plasmids were purchased from MIAOLING BIOLOGY ( http://www.miaolingbio.com/ ), and the ALYREF and YBX1 fractions were then amplified using specific primer. .. The ALYREF and YBX1 portions were amplified using Plasmid Preparation:Article Title: Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing Article Snippet: ALYREF and YBX1 expression plasmids were purchased from MIAOLING BIOLOGY ( http://www.miaolingbio.com/ ), and the ALYREF and YBX1 fractions were then amplified using specific primer. .. The ALYREF and YBX1 portions were amplified using Article Title: Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing Article Snippet: ALYREF and YBX1 expression plasmids were purchased from MIAOLING BIOLOGY ( http://www.miaolingbio.com/ ), and the ALYREF and YBX1 fractions were then amplified using specific primer. .. The ALYREF and YBX1 portions were amplified using Construct:Article Title: Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing Article Snippet: ALYREF and YBX1 expression plasmids were purchased from MIAOLING BIOLOGY ( http://www.miaolingbio.com/ ), and the ALYREF and YBX1 fractions were then amplified using specific primer. .. The ALYREF and YBX1 portions were amplified using Article Title: Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing Article Snippet: ALYREF and YBX1 expression plasmids were purchased from MIAOLING BIOLOGY ( http://www.miaolingbio.com/ ), and the ALYREF and YBX1 fractions were then amplified using specific primer. .. The ALYREF and YBX1 portions were amplified using Cloning:Article Title: Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing Article Snippet: ALYREF and YBX1 expression plasmids were purchased from MIAOLING BIOLOGY ( http://www.miaolingbio.com/ ), and the ALYREF and YBX1 fractions were then amplified using specific primer. .. The ALYREF and YBX1 portions were amplified using Article Title: Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing Article Snippet: ALYREF and YBX1 expression plasmids were purchased from MIAOLING BIOLOGY ( http://www.miaolingbio.com/ ), and the ALYREF and YBX1 fractions were then amplified using specific primer. .. The ALYREF and YBX1 portions were amplified using |